4D printing of extracellular matrix

pilot project for the development of complex tissues

Authors

  • Heloisa Moreira Feijó Faculdade de Medicina de Olinda (FMO)
  • Mariana Lima de Morais Inocêncio Faculdade de Medicina de Olinda (FMO)
  • Laiz Correia Arruda Faculdade de Medicina de Olinda (FMO)
  • Vitor Benedito Ferreira Freire Faculdade de Medicina de Olinda (FMO)
  • Edilson Eugênio da Silva Instituto Federal de Pernambuco (IFPE)
  • Frederico Duarte de Menezes Instituto Federal de Pernambuco (IFPE)
  • Jacek Stanislaw Michewicz Instituto Federal de Pernambuco (IFPE)
  • José Ângelo Peixoto da Costa Instituto Federal de Pernambuco (IFPE)
  • Luciana Lima Monteiro Instituto Federal de Pernambuco (IFPE)
  • Clessio Leão Silva Lima Universidade Federal de Pernambuco (UFPE)
  • João Victor Barreto Ipiranga Universidade Federal de Pernambuco (UFPE)
  • Amaury de Siqueira Medeiros Filho 3D Bladder Printing Workgroup
  • Jorge Eduardo Oliveira Filho 3D Bladder Printing Workgroup
  • Amanda Vasconcelos de Albuquerque Faculdade de Medicina de Olinda (FMO)
  • Leonardo Lima Monteiro fro

DOI:

https://doi.org/10.56102/afmo.2022.196

Keywords:

Bioprinting, Extracellular Matrix, Regenerative Medicine, Bioengineering, Scaffolds

Abstract

Introduction: The shortage of organs and tissues for transplantation to meet the existing demand is one of the great challenges in the medical field. A feasible solution to this problem is the three-dimensional (3D) printing of organs and tissues. The present study presents preliminary results of the first 4D extracellular matrix (ECM) models using a biomaterial.

Methods: Experimental, multicenter study carried out at the Simulation and Digital Fabrication Laboratory (SIMUFAB) of the Federal Institute of Pernambuco (IFPE). A 2% sodium alginate solution was used to manufacture the MEC on a Zmorph 3D printer, type FDM.

Results: The MEC geometric figure was a square with an area of ​​1cm2 and developed by the Voxelizer software, initially designed in CAD (computer aided design). Next, the biomaterial in gel form was printed with an extrusion speed of 1mm/s and a printing speed of 3mm/s. We succeed in the printing with the biopolymer selected for the manufacture of MEC. There was a uniform deposit of the material during printing, which allowed obtaining the geometric shape designed in CAD.

Discussion: This article shows preliminary results of the printing process of a 4D MEC. The achievement of the MEC impression qualifies alginate as one of the main biopolymers to be used in the future phases of this study.

Conclusion: The variation of the nozzle area and the extrusion speed have a great influence on the analysis of the final parameters to use the 3D printer.

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Published

2022-12-22

How to Cite

Moreira Feijó, H. ., Lima de Morais Inocêncio, M. ., Correia Arruda, L. ., Benedito Ferreira Freire, V. ., Eugênio da Silva, E., Duarte de Menezes, F. ., Stanislaw Michewicz, J. ., Peixoto da Costa, J. Ângelo ., Lima Monteiro, L. ., Leão Silva Lima, C., Barreto Ipiranga, J. V. ., de Siqueira Medeiros Filho, A. ., Eduardo Oliveira Filho, J. ., Vasconcelos de Albuquerque, A. ., & Lima Monteiro, L. (2022). 4D printing of extracellular matrix: pilot project for the development of complex tissues. Annals of Olinda Medical School, 1(7), 7–13. https://doi.org/10.56102/afmo.2022.196

Issue

Vol. 1 No. 7 (2022)

Section

Original Articles

License

Copyright (c) 2022 Leonardo Lima Monteiro, Heloisa Moreira Feijó, Mariana Lima de Morais Inocêncio, Laiz Correia Arruda, Vitor Benedito Ferreira Freire, Edilson Eugênio da Silva, Frederico Duarte de Menezes, Jacek Stanislaw Michewicz, José Ângelo Peixoto da Costa, Luciana Lima Monteiro, Clessio Leão Silva Lima, João Victor Barreto Ipiranga, Amaury de Siqueira Medeiros Filho, Jorge Eduardo Oliveira Filho, Amanda Vasconcelos de Albuquerque

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