Microbiological analysis of chitosan membranes with n-acylydrazone associations

evaluation of efficiency

Authors

DOI:

https://doi.org/10.56102/afmo.2024.322

Keywords:

Chitosan, Healing, Membranes, Wounds

Abstract

Objective: To verify the potential and bactericidal activity of chitosan (CS) films combined with N-acylhydrazone (JR19) in different concentrations for wound treatment. Methodology: Films were developed from chitosan (CS) + polyvinyl acetate (PVA) solutions. The F1 film consisted of CS and the F2 film consisted of CS + PVA in a 1:1 ratio. To incorporate JR19 in the polymeric solutions, 1 mg of sulfadiazine (AgSD) + 8.6 mg of JR19 were added in 10 mL of CS: PVA. Films formed from this solution were named F3, F4, and F5. The antimicrobial activity of films with JR19 derivatives was assessed using the disc diffusion technique in an Agar Müeller Hinton solid culture medium. Results: The F1 film was discarded due to strong electrostatic repulsion with solid agar culture medium, deforming the film and hampering the culture. The F2, F3, and F5 films showed bacterial colony formations in front and verse, indicating no microbial inhibitory activity on Staphylococcus aureus. Conclusion: In the F4 film, the synergy between JR19 and AgSD showed complementary properties that facilitate wound healing. However, the safety and efficacy of this alternative therapy need further investigation in clinical trials.

Author Biographies

Brenda Beatriz Torres de Oliveira, Faculdade de Medicina de Olinda

Student at the Faculdade de Medicina de Olinda. Olinda, Pernambuco, Brazil.

Maria Eduarda Alves da Nóbrega, Faculdade de Medicina de Olinda

Student at the Faculdade de Medicina de Olinda. Olinda, Pernambuco, Brazil.

Camila Ananias Bezerra de Lima, Faculdade de Medicina de Olinda

Student at the Faculdade de Medicina de Olinda. Olinda, Pernambuco, Brazil.

Gabryella Garcia Guedes, Universidade Estadual da Paraíba

Master's student at the Universidade Estadual da Paraíba. Campina Grande, Paraíba, Brazil.

Luciana Ramos Teixeira, Faculdade de Medicina de Olinda

Professor at the Faculdade de Medicina de Olinda. Olinda, Pernambuco, Brazil.

Bolívar Ponciano Goulart de Lima Damasceno, Universidade Estadual da Paraíba

Possui graduação em Farmácia, mestrado em Ciências Farmacêuticas e doutorado em Ciências da Saúde pela Universidade Federal do Rio Grande do Norte. É Professor Associado nível B RETIDE da Universidade Estadual da Paraíba e coordenador do Laboratório de Desenvolvimento e Caracterização de Produtos Farmacêuticos. Tem experiência na área de Farmácia, com ênfase em Farmacotécnica, Tecnologia e Nanotecnologia Farmacêuticas, atuando principalmente nos seguintes temas: microemulsão, micro/nanopartículas e filmes poliméricos como sistemas transportadores de fármacos; e na área da Biotecnologia na extração de polímeros naturais (celulose) de plantas cactáceas e sisal. Atualmente, é professor permanente e coordenador adjunto do Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Estadual da Paraíba, professor permanente do Programa de Pós-Graduação em Desenvolmento e Inovação Tecnológica em Medicamentos da Universidade Federal da Paraíba.

Tharcia Kiara Beserra de Oliveira, Faculdade de Medicina de Olinda

Doutora em Engenharia Agrícola pela Universidade Federal de Campina Grande; Mestre em Engenharia Agrícola na Área de Processamento e Armazenamento de Produtos Agrícolas; Especialização em Auditoria em Saúde pelo Centro de Ensino Superior e Desenvolvimento; Graduação em Medicina Veterinária pela Universidade Federal de Campina Grande. Professora da Faculdade de Medicina de Olinda e do Centro Universitário UNIFACISA. Tem experiência na área de Medicina Veterinária, atuando principalmente no seguinte tema: Biotério, Produtos e Processos Aplicados a Alimentos, Animais de Laboratório, Ética e Bem-Estar Animal.

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Published

2024-12-19

How to Cite

Oliveira, B. B. T. de, Nóbrega, M. E. A. da, Lima, C. A. B. de, Guedes, G. G., Teixeira, L. R., Damasceno, B. P. G. de L., & Oliveira, T. K. B. de. (2024). Microbiological analysis of chitosan membranes with n-acylydrazone associations: evaluation of efficiency. Annals of Olinda Medical School, 1(12), 56–65. https://doi.org/10.56102/afmo.2024.322

Issue

Vol. 1 No. 12 (2024)

Section

Original Articles

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Copyright (c) 2024 Brenda Beatriz Torres de Oliveira, Maria Eduarda Alves da Nóbrega, Camila Ananias Bezerra de Lima, Gabryella Garcia Guedes, Luciana Ramos Teixeira, Bolívar Ponciano Goulart de Lima Damasceno, Tharcia Kiara Beserra de Oliveira

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