Microbiological analysis of chitosan polymer membranes associated with Schinus terebinthifolius for treating skin wounds
DOI:
https://doi.org/10.56102/afmo.2025.407Keywords:
Chitosan, Plant extracts, Schinus, Wound healingAbstract
Objective: To develop and evaluate chitosan membranes containing Schinus terebinthifolius bark extract for topical use in skin lesions. Methods: Chitosan membranes were prepared with different concentrations of Schinus terebinthifolius extract. The resistance to folding, the pH of the membranes, and the antimicrobial activity of the extract were evaluated using the disc diffusion technique on Mueller-Hinton agar. The bacterial inoculum used was Staphylococcus aureus ATCC® 25923. The diameters of the inhibition zones were measured with a caliper to determine the sensitivity of the bacteria. The interpretation of the results followed the cutoff points for minimum inhibitory concentration (MIC) and halo diameters from the Brazilian Committee on Antimicrobial Susceptibility Testing. Results: After incubation at 30°C for 24 hours, the Schinus terebinthifolius extract at concentrations of 43.0 µg and 21.5 µg showed the best inhibition zones for Staphylococcus aureus (16 mm and 14 mm, respectively). The membrane with 5% extract exhibited greater resistance to folding, supporting more than 300 folds before breaking. In contrast, the 10% membrane became very rigid and had low adhesion after only 12 folds. The pH of the membranes was 5.5 in both concentrations, without a significant difference. Conclusion: The best antimicrobial results from Schinus terebinthifolius extract were at concentrations of 43.0 µg and 21.5 µg. The membrane with 5% of the extract showed the best adaptation, while the one with 10% became excessively rigid. Further studies must explore lower concentrations of the extract.
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Copyright (c) 2025 Sarah Maria Lucena Teles Cruz, Gislaine Simões Portela Barros, Leane de Fátima Matias do Nascimento, Camila Ananias Bezerra de Lima, Gabryella Garcia Guedes, Luciana Ramos Teixeira, Tharcia Kiara Beserra de Oliveira
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